Open AccessPhase shifting technique for extended inline holographic microscopy with a pinhole array
Author(s) -
Christian Graulig,
Mario Kanka,
Rainer Riesenberg
Publication year - 2012
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.20.022383
Subject(s) - pinhole (optics) , optics , holography , digital holographic microscopy , microscopy , phase (matter) , materials science , sample (material) , physics , quantum mechanics , thermodynamics
Digital inline holographic microscopy using a pinhole for sample illumination allows lensless imaging. To overcome restrictions of the sample size and density in the setup additional reference waves are generated by extending the single pinhole to a regular 2D pinhole array illumination. A technique is presented that uses phase shifting between the pinhole waves. Multiple foci with stable phase differences and a phase error (rms) of 0.027 rad generate pinhole waves which illuminate an undiluted, dense blood smear sample. Amplitude and phase images of the blood sample were successfully reconstructed.