Open AccessPhase contrast microscopy with fringe contrast adjustable by using grating-based phase-shifter
Author(s) -
Juanjuan Zheng,
Baoli Yao,
Peng Gao,
Tong Ye
Publication year - 2012
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.20.016077
Subject(s) - optics , grating , contrast (vision) , spatial frequency , diffraction , phase (matter) , diffraction grating , phase modulation , phase contrast imaging , blazed grating , spatial light modulator , diffraction efficiency , materials science , physics , phase contrast microscopy , phase noise , quantum mechanics
In this paper a new phase contrast method with fringe contrast adjustable is proposed. In the Fourier plane of the object wave, two Ronchi gratings i.e., a central grating and a surrounding grating, are used to modulate the phases of the undiffracted and diffracted components, respectively. By loading the two gratings separately on spatial light modulator, the undiffracted and diffracted components can be measured independently, which simplify greatly the reconstruction process. Besides, the fringe contrast of the phase contrast interferogram can be adjusted by changing the modulation depth of the two gratings. The feasibility of the proposed method is verified by theoretical analysis and experiment.