Multi-contrast focal modulation microscopy for in vivo imaging of thick biological tissues | Zendy

Nanguang Chen | Zendy; Guangjun Gao | Zendy

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Multi-contrast focal modulation microscopy for in vivo imaging of thick biological tissues

Author(s) -

Nanguang Chen,

Guangjun Gao

Publication year - 2012

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.20.012166

Subject(s) - optics , microscopy , microscope , biological imaging , materials science , optical coherence tomography , fluorescence microscope , biological specimen , preclinical imaging , two photon excitation microscopy , fluorescence lifetime imaging microscopy , image resolution , phase modulation , spatial light modulator , light sheet fluorescence microscopy , scanning confocal electron microscopy , fluorescence , in vivo , physics , phase noise , microbiology and biotechnology , biology

In vivo high resolution imaging of biological tissues is desirable for a wide range of biomedical applications. Recently focal modulation microscopy (FMM) has been developed and an imaging depth comparable to multi-photon microscopy (MPM) and optical coherence microscopy (OCM) has been achieved. Here we report the first focal modulation microscope that is capable of performing real-time fluorescence and scattering imaging simultaneously on thick biological tissues. A novel spatiotemporal phase modulator (STPM) has been designed and integrated into such a microscope to achieve high performances in terms of imaging speed, contrast, effective spatial resolution, signal to noise ratio, and compatibility with multiple excitation wavelengths.

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