Open AccessMulti-contrast focal modulation microscopy for in vivo imaging of thick biological tissues
Author(s) -
Nanguang Chen,
Ge Gao
Publication year - 2012
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.20.012166
Subject(s) - optics , microscopy , microscope , biological imaging , materials science , optical coherence tomography , fluorescence microscope , biological specimen , preclinical imaging , two photon excitation microscopy , fluorescence lifetime imaging microscopy , image resolution , phase modulation , spatial light modulator , light sheet fluorescence microscopy , scanning confocal electron microscopy , fluorescence , in vivo , physics , phase noise , microbiology and biotechnology , biology
In vivo high resolution imaging of biological tissues is desirable for a wide range of biomedical applications. Recently focal modulation microscopy (FMM) has been developed and an imaging depth comparable to multi-photon microscopy (MPM) and optical coherence microscopy (OCM) has been achieved. Here we report the first focal modulation microscope that is capable of performing real-time fluorescence and scattering imaging simultaneously on thick biological tissues. A novel spatiotemporal phase modulator (STPM) has been designed and integrated into such a microscope to achieve high performances in terms of imaging speed, contrast, effective spatial resolution, signal to noise ratio, and compatibility with multiple excitation wavelengths.