Open AccessShort time behavior of fluorescence intensity fluctuations in single molecule polarization sensitive experiments
Author(s) -
Lior Turgeman,
Dror Fixler
Publication year - 2012
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.20.009276
Subject(s) - polarization (electrochemistry) , optics , fluorescence , single molecule experiment , rotational diffusion , molecule , photon , physics , photon counting , molecular physics , fluorescence anisotropy , materials science , atomic physics , chemistry , anisotropy , quantum mechanics
Recent developments in the field of single molecule orientation imaging have led us to devise a simple framework for analyzing fluorescence intensity fluctuations in single molecule polarization sensitive experiments. Based on the new framework, rotational dynamics of individual molecules are quantified, in this paper, from the short time behavior of the time averaged fluorescence intensity fluctuation trajectories. The suggested model can be applied in single molecule fluorescence fluctuations experiments to extract accurate expectation values of photon counts during very short integration time in which rotational diffusion is likely not to be averaged out.