Open AccessVertical optical sectioning using a magnetically driven confocal microscanner aimed for in vivo clinical imaging
Author(s) -
Hadi Mansoor,
Haishan Zeng,
Keqin Chen,
Yingqiu Yu,
Jianhua Zhao,
Mu Chiao
Publication year - 2011
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.025161
Subject(s) - materials science , optics , confocal , microlens , optical sectioning , confocal microscopy , laser , wafer , optical axis , optical fiber , surface micromachining , transverse plane , optoelectronics , lens (geology) , physics , medicine , alternative medicine , structural engineering , pathology , fabrication , engineering
This paper presents a confocal microscanner for direct vertical optical sectioning of biological samples. Confocal imaging is performed by transverse (X-axis) and axial (Z-axis) scanning of a focused laser beam using an optical fiber and a microlens respectively. The actuators are fabricated by laser micromachining techniques and are driven by electromagnetic forces. Optical and mechanical performance of the system is predicted by simulation software packages and characterized by experimental measurements. The scanner has lateral resolution of 3.87 µm and axial resolution of 10.68 µm with a field of view of 145 µm in X and 190 µm in Z directions. Confocal imaging of a polymer layer deposited on a silicon wafer and onion epidermal cells is demonstrated.