Open AccessThree dimension optical imaging in a high throughput layered expression system
Author(s) -
Reut Cohen,
Gallya Gannot,
Moshe Ben David,
Nir Sochen,
Israel Gannot
Publication year - 2011
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.019822
Subject(s) - stack (abstract data type) , throughput , multiplex , computer science , optics , visualization , proof of concept , imaging phantom , matlab , dimension (graph theory) , process (computing) , grid , materials science , artificial intelligence , physics , bioinformatics , telecommunications , mathematics , pure mathematics , wireless , biology , programming language , operating system , geometry
Layered peptide array (LPA) system enables multiplex screening of biomarkers [1-3]. One of the main problems of the LPA system is the screening of the layered-membranes stack. Nowadays, each membrane is imaged separately using conventional fluorescent imaging. This process is time consuming and requires extensive manual interaction. This paper describes a general solution for optical imaging of a layered grid medium using photogrammetric methods. The suggested method enables visualization of the LPA membranes stack by using only two images of the stack. This study is a proof of concept of the suggested solution using MATLAB simulation and phantom experiments.