Open AccessMultiplex spectral surface plasmon resonance imaging (SPRI) sensor based on the polarization control scheme
Author(s) -
Chi Lok Wong,
George Chung Kit Chen,
Beng Koon Ng,
Shuchi Agarwal,
Zhiping Lin,
Peng Chen,
Ho Pui Ho
Publication year - 2011
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.018965
Subject(s) - multiplex , surface plasmon resonance , refractive index , optics , materials science , detection limit , spectral imaging , polarization (electrochemistry) , spectral resolution , surface plasmon , plasmon , optoelectronics , spectral line , chemistry , physics , nanotechnology , nanoparticle , bioinformatics , chromatography , astronomy , biology
A two-dimensional (2D) spectral SPR sensor based on a polarization control scheme is reported in this paper. The polarization control configuration converts the phase difference between p- and s- polarization occurring at surface plasmon resonance (SPR) into corresponding color responses in spectral SPR images. A sensor resolution of 2.7 x 10(-6) RIU has been demonstrated, which corresponds to more than one order of magnitude resolution improvement (26 times) comparing to existing 2D spectral SPR sensors. Multiplex array detection has also been demonstrated with the spectral SPR imaging sensor. In a 8 x 4 sensor array, 32 samples with different refractive index values were monitored simultaneously. Detection on bovine serum albumin (BSA) antigen-antibody binding further demonstrated the multiplex detection capability of the 2D spectral SPR sensor for bio-molecular interactions. The detection limit is found to be 21 ng/ml, which is 36 times better than the detection limit previously reported by phase imaging SPR sensors. In light of the advantages of high sensitivity, 2D multiplex detection and real-time response, the spectral SPR imaging sensor can find promising applications in rapid, high throughput, non-labeling and multiplex detection of protein array for proteomics studies, biomarker screening, disease prognosis, and drug discovery.