Open AccessMultivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy
Author(s) -
Zachary J. Smith,
Sven Strombom,
Sebastian WachsmannHogiu
Publication year - 2011
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.016950
Subject(s) - digital micromirror device , optics , spectrograph , photomultiplier , materials science , raman spectroscopy , detector , fluorescence , fluorescence spectroscopy , spectroscopy , raman scattering , principal component analysis , multivariate statistics , optoelectronics , spectral line , physics , computer science , quantum mechanics , astronomy , artificial intelligence , machine learning
A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells.