Open AccessWavelength encoding technique for particle analyses in hematology analyzer
Author(s) -
Nelly Rongeat,
Pascal Brunel,
Jean-Philippe Gineys,
Didier Cremien,
Vincent Couderc,
Philippe Nérin
Publication year - 2011
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.014076
Subject(s) - optics , wavelength , hematology analyzer , spectrum analyzer , fluorescence , materials science , laser , excitation wavelength , compensation (psychology) , physics , medicine , psychology , pathology , psychoanalysis
The aim of this study is to combine multiple excitation wavelengths in order to improve accuracy of fluorescence characterization of labeled cells. The experimental demonstration is realized with a hematology analyzer based on flow cytometry and a CW laser source emitting two visible wavelengths. A given optical encoding associated to each wavelength allows fluorescence identification coming from specific fluorochromes and avoiding the use of noisy compensation method.