Wavelength encoding technique for particle analyses in hematology analyzer | Zendy

Nelly Rongeat | Zendy; Patrick Brunel | Zendy; Jean-Philippe Gineys | Zendy; Didier Cremien | Zendy; Vincent Couderc | Zendy; Philippe Nérin | Zendy

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Wavelength encoding technique for particle analyses in hematology analyzer

Author(s) -

Nelly Rongeat,

Patrick Brunel,

Jean-Philippe Gineys,

Didier Cremien,

Vincent Couderc,

Philippe Nérin

Publication year - 2011

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.19.014076

Subject(s) - optics , wavelength , hematology analyzer , spectrum analyzer , fluorescence , materials science , laser , excitation wavelength , compensation (psychology) , physics , medicine , psychology , pathology , psychoanalysis

The aim of this study is to combine multiple excitation wavelengths in order to improve accuracy of fluorescence characterization of labeled cells. The experimental demonstration is realized with a hematology analyzer based on flow cytometry and a CW laser source emitting two visible wavelengths. A given optical encoding associated to each wavelength allows fluorescence identification coming from specific fluorochromes and avoiding the use of noisy compensation method.

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