Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures | Zendy

Keng-Chi Cho | Zendy; Chi-Hsiang Lien | Zendy; ChunYu Lin | Zendy; Chia-Yuan Chang | Zendy; Lynn L. H. Huang | Zendy; Paul J. Campagnola | Zendy; Dong Chen | Zendy; Shean-Jen Chen | Zendy

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Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

Author(s) -

Keng-Chi Cho,

Chi-Hsiang Lien,

ChunYu Lin,

Chia-Yuan Chang,

Lynn L. H. Huang,

Paul J. Campagnola,

Dong Chen,

Shean-Jen Chen

Publication year - 2011

Publication title -

optics express

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.19.011732

Subject(s) - bovine serum albumin , materials science , rose bengal , femtosecond , microstructure , fluorescence , optics , two photon excitation microscopy , laser , analytical chemistry (journal) , chemistry , chromatography , composite material , physics , organic chemistry

In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.

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