
Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures
Author(s) -
Keng Chi Cho,
Chi-Hsiang Lien,
Chun Yu Lin,
Chia Yuan Chang,
Lynn Ling-Huei Huang,
Paul J. Campagnola,
Dong Chen,
Shean Jen Chen
Publication year - 2011
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.011732
Subject(s) - bovine serum albumin , materials science , rose bengal , femtosecond , microstructure , fluorescence , optics , two photon excitation microscopy , laser , analytical chemistry (journal) , chemistry , chromatography , composite material , physics , organic chemistry
In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.