Open AccessBlood cell assisted in vivo Particle Image Velocimetry using the confocal laser scanning microscope
Author(s) -
Sung Min Choi,
Wi Han Kim,
Daniel Côté,
Cheolwoo Park,
Ho Lee
Publication year - 2011
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.004357
Subject(s) - confocal , confocal microscopy , microscope , particle image velocimetry , velocimetry , particle tracking velocimetry , optics , materials science , microscopy , in vivo , biomedical engineering , blood flow , capillary action , physics , biology , medicine , turbulence , microbiology and biotechnology , composite material , thermodynamics
We demonstrated the feasibility of blood cell assisted in vivo Particle Image Velocimetry using confocal microscopy. Blood flow of skin vessel in a mouse was non-invasively imaged in vivo using a confocal microscopy. The video-rate confocal microscope was used to monitor the motion of the blood cells in the capillary of a live mouse ear. The home-built confocal laser scanning microscopy allowed us to take images at the acquisition rate of 30 frames per second. The individual blood cells could be distinguished from other cells and the trajectory of the each cell could be followed in the sequential images. The acquired confocal images were used to get the velocity profile of the in vivo blood flow in conjunction with the Particle Image Velocimetry (PIV), without injecting any exogenous nano/micro particles into the mouse. We were able to measure the blood velocity up to a few hundreds µm/sec for various vessels in a live mouse. Because there is no need for the injection of the exogenous tracing particles, it is expected that we could apply the current technology to the study of human capillary blood stream.