Open AccessCoherent noise reduction in digital holographic phase contrast microscopy by slightly shifting object
Author(s) -
Feng Pan,
Wen Xiao,
Shuo Liu,
Fanjing Wang,
Lü Rong,
Rui Li
Publication year - 2011
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.003862
Subject(s) - digital holographic microscopy , optics , digital holography , holography , phase (matter) , noise (video) , noise reduction , physics , spatial frequency , speckle noise , microscopy , parametric statistics , image plane , computer science , artificial intelligence , mathematics , speckle pattern , image (mathematics) , acoustics , statistics , quantum mechanics
A method to reduce coherent noise in digital holographic phase contrast microscopy is proposed. By slightly shifting the specimen, a series of digital holograms with different coherent noise patterns is recorded. Each hologram is reconstructed individually, while the different phase tilts of the reconstructed complex amplitudes due to the specimen shifts are corrected in the hologram plane by using numerical parametric lens method. Afterward, the lateral displacements of the phase maps from different holograms are compensated in the image plane by using digital image registration method. Thus, all phase images have same distribution, but uncorrelated coherent noise patterns. By a proper averaging procedure, the coherent noise of phase contrast image is reduced significantly. The experimental results are given to confirm the proposed method.