Open AccessIntegrated spectrometer design with application to multiphoton microscopy
Author(s) -
Eric V. Chandler,
Charles G. Durfee,
Jeffrey A. Squier
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.19.000118
Subject(s) - optics , spectrometer , microscope , microscopy , polarization (electrochemistry) , materials science , imaging spectrometer , excitation , detector , spectral imaging , physics , chemistry , quantum mechanics
We present a prism-based spectrometer integrated into a multifocal, multiphoton microscope. The multifocal configuration facilitates interrogation of samples under different excitation conditions. Notably, the image plane of the microscope and the image plane of the spectrometer are coincident eliminating the need for an intermediate image plane containing an entrance slit. An EM-CCD detector provides sufficient gain for spectral interrogation of single-emitters. We employ this spectrometer to observe spectral shifts in the two-photon excitation fluorescence emission of single CdSe nanodots as a function of excitation polarization.