Open AccessExperimental Cerenkov luminescence tomography of the mouse model with SPECT imaging validation
Author(s) -
Zhenhua Hu,
Jimin Liang,
Weidong Yang,
Weiwei Fan,
Congye Li,
Xiaolong Ma,
Xueli Chen,
Xiaolong Ma,
Xiangsi Li,
Xiaobo Qu,
Jing Wang,
Feng Cao,
Hui Hui
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.024441
Subject(s) - single photon emission computed tomography , molecular imaging , preclinical imaging , tomography , optics , emission computed tomography , spect imaging , physics , luminescence , positron emission tomography , nuclear medicine , in vivo , medicine , microbiology and biotechnology , biology
Optical molecular imaging resulting from Cerenkov radiation has become a motivating topic recently and will potentially open new avenues for the study of small animal imaging. Cerenkov-based optical imaging taken from living animals in vivo has been studied with two-dimensional (2D) planar geometry and three-dimensional (3D) homogeneous mouse model. In this study, we performed 3D Cerenkov-based luminescence tomography (CLT) using a heterogeneous mouse model with an implanted Na(131)I radioactive source, which provided the accurate location for the reconstructed source. Furthermore, single photon emission computed tomography (SPECT) was utilized to verify the results of 3D CLT. We reconstructed the localization and intensity of an embedded radioactive source with various concentrations, and established a quantitative relationship between the radiotracer activity and the reconstructed intensity. The results showed the ability of in vivo CLT to recover the radioactive probe distribution in the heterogeneous mouse model and the potential of a SPECT imaging validation strategy to verify the results of optical molecular tomography.