Open AccessPhotophysical mechanisms of collagen modification by 80 MHz femtosecond laser
Author(s) -
Vladimir A. Hovhannisyan,
Ara Ghazaryan,
YungFang Chen,
Shean-Jen Chen,
Dong Chen
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.024037
Subject(s) - femtosecond , autofluorescence , laser , materials science , optics , sapphire , ti:sapphire laser , second harmonic generation , optoelectronics , laser power scaling , fluorescence , excitation , physics , quantum mechanics
Photophysical mechanisms of collagen photomodification (CFP) by the use of a 80 MHz, 780 nm femtosecond titanium-sapphire laser were investigated. Our observation that the decrease in collagen second harmonic generation and increase in two-photon autofluorescence intensity occurred primarily at sites where photoproducts were present suggested that the photoproducts may act to facilitate the CFP process. Laser power study of CFP indicated that the efficiency of the process depended on the sixth power of the laser intensity. Furthermore, it was demonstrated that CFP can be used for bending and cutting of collagen fibers and creating 3D patterns within collagen matrix with high precision (~2 μm).