Open AccessAngular domain fluorescence lifetime imaging: a tissue-like phantom study
Author(s) -
Mohamadreza Najiminaini,
Fartash Vasefi,
Kenneth M. Tichauer,
TingYim Lee,
Bożena Kaminska,
Jeffrey J. L. Carson
Publication year - 2010
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.023247
Subject(s) - pinhole (optics) , optics , imaging phantom , collimated light , lens (geology) , fluorescence , angular resolution (graph drawing) , image resolution , fluorescence lifetime imaging microscopy , materials science , physics , laser , mathematics , combinatorics
We describe a fluorescence lifetime imaging technique employing the collimation detection capabilities of an angular filter array (AFA). The AFA accepts minimally scattered photons emitted from fluorophores up to 2 mm deep within turbid media. The technique, referred to as Angular Domain Fluorescence Lifetime Imaging (ADFLI), is described and its performance evaluated in comparison to a conventional (lens and pinhole) system. Results from a tissue-mimicking phantom demonstrated that ADFLI provides better spatial resolution and image contrast for fluorescent probes at greater depths compared to a lens and pinhole system.