Open AccessStudy of cell-biosubstrate contacts via surface plasmon polariton phase microscopy
Author(s) -
Yuan Deng Su,
KueiChen Chiu,
NanShan Chang,
HuaLin Wu,
Shean Jen Chen
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.020125
Subject(s) - surface plasmon polariton , surface plasmon resonance , materials science , microscopy , optics , phase (matter) , surface plasmon , phase imaging , contact angle , plasmon , nanoparticle , optoelectronics , nanotechnology , chemistry , physics , organic chemistry , composite material
This study utilized a developed surface plasmon polariton (SPP) phase microscopy to observe cell-biosubstrate contacts. The developed SPP phase microscopy is highly sensitive to cell membrane contact with biosubstrates and also provides long-term phase stability to achieve time-lapse living cell observation. As such, an SPP intensity and phase sensitivity comparison demonstrates that the sensitivity of the phase measurement can be 100-fold greater than that of the intensity measurement. Also, a more than 2-hour cell apoptosis observation via the SPP phase microscopy is presented. To implement the incident angle from 70° to 78°, cell-biosubstrate contact images corresponding to the surface plasmon resonance (SPR) angles are obtained by utilizing the SPP phase measurement. According to the information of the corresponding SPR angle image and a multilayer simulation, the contact distances between a living melanoma cell and a bovine serum albumin substrate at four different locations have been estimated.