Open AccessA method for achieving super-resolved widefield CARS microscopy
Author(s) -
Kim M. Hajek,
Brad Littleton,
D. J. Turk,
Timothy J. McIntyre,
Halina RubinszteinDunlop
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.019263
Subject(s) - optics , diffraction , raman scattering , physics , image resolution , microscopy , scattering , superresolution , computer science , resolution (logic) , raman spectroscopy , computer vision , artificial intelligence , image (mathematics)
We propose a scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy images with sub-diffraction-limited resolution. This approach adds structured illumination to the widefield CARS configuration [Applied Physics Letters 84, 816 (2004)]. By capturing a number of images at different phases of the standing wave pattern, an image with up to three times the resolution of the original can be constructed. We develop a theoretical treatment of this system and perform numerical simulations for a typical CARS system, which indicate that resolutions around 120 nm are obtainable with the present scheme. As an imaging system, this method combines the advantages of sub-diffraction-limited resolution, endogenous contrast generation, and a wide field of view.