Open AccessEfficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy
Author(s) -
Alexandre Kudlinski,
Mickaël Lelek,
Benoît Barviau,
Laurent Audry,
Arnaud Mussot
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.016640
Subject(s) - supercontinuum , biophotonics , materials science , optics , microscopy , photonic crystal fiber , photonic crystal , optoelectronics , laser , fluorescence microscope , fiber laser , fluorescence , photonics , wavelength , physics
Using a low-cost microchip laser and a long photonic crystal fiber taper, we report a supercontinuum source with a very efficient visible conversion, especially in the blue region (around 420 nm). About 30 % of the total average output power is located in the 350-600 nm band, which is of primary importance in a number of biophotonics applications such as flow cytometry or fluorescence imaging microscopy for instance. We successfully demonstrate the use of this visible-enhanced source for a three-color imaging of HeLa cells in wide-field microscopy.