Efficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy | Zendy

Alexandre Kudlinski | Zendy; Mickaël Lelek | Zendy; B. Barviau | Zendy; Laurent Audry | Zendy; Arnaud Mussot | Zendy

Open Access

Efficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy

Author(s) -

Alexandre Kudlinski,

Mickaël Lelek,

B. Barviau,

Laurent Audry,

Arnaud Mussot

Publication year - 2010

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.18.016640

Subject(s) - supercontinuum , biophotonics , materials science , optics , microscopy , photonic crystal fiber , photonic crystal , optoelectronics , laser , fluorescence microscope , fiber laser , fluorescence , photonics , wavelength , physics

Using a low-cost microchip laser and a long photonic crystal fiber taper, we report a supercontinuum source with a very efficient visible conversion, especially in the blue region (around 420 nm). About 30 % of the total average output power is located in the 350-600 nm band, which is of primary importance in a number of biophotonics applications such as flow cytometry or fluorescence imaging microscopy for instance. We successfully demonstrate the use of this visible-enhanced source for a three-color imaging of HeLa cells in wide-field microscopy.

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