Open AccessQuantitative SLM-based differential interference contrast imaging
Author(s) -
Timothy J. McIntyre,
Christian Maurer,
Stephanie Fassl,
Saranjam Khan,
Stefan Bernet,
Monika RitschMarte
Publication year - 2010
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.014063
Subject(s) - differential interference contrast microscopy , optics , spatial light modulator , materials science , interference (communication) , spatial frequency , microscopy , optical path , phase contrast imaging , differential phase , image resolution , fourier transform , phase (matter) , computer science , phase contrast microscopy , physics , telecommunications , channel (broadcasting) , quantum mechanics
We describe the implementation of quantitative Differential Interference Contrast (DIC) Microscopy using a spatial light modulator (SLM) as a flexible Fourier filter in the optical path. The experimental arrangement allows for the all-electronic acquisition of multiple phase shifted DIC-images at video rates which are analyzed to yield the optical path length variation of the sample. The resolution of the technique is analyzed by retrieving the phase profiles of polystyrene spheres in immersion oil, and the method is then applied for quantitative imaging of biological samples. By reprogramming the diffractive structure displayed at the SLM it is possible to record the whole set of phase shifted DIC images simultaneously in different areas of the same camera chip. This allows for quantitative snap-shot imaging of a sample, which has applications for the investigation of dynamic processes.