Open AccessInfrared imaging of an A549 cultured cell by a vibrational sum-frequency generation detected infrared super-resolution microscope
Author(s) -
Satoshi Kogure,
Keiichi Inoue,
T. Ohmori,
Miya Ishihara,
Makoto Kikuchi,
Masaaki Fujii,
Makoto Sakai
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.013402
Subject(s) - infrared , microscope , optics , resolution (logic) , materials science , microscopy , infrared microscopy , infrared spectroscopy , wavelength , image resolution , chemistry , physics , organic chemistry , artificial intelligence , computer science
We performed infrared (IR) spectroscopic imaging of molecular species in cultured cell interiors of A549 cells using in-house developed vibrational sum-frequency generation detected IR super-resolution microscope. The spatial resolution of this IR microscope was approximately 1.1 microm, which exceeds the diffraction limit of IR light. Therefore, we clearly observed differences in the signal intensity at various IR wavelengths which appear to originate from the differing IR absorptions of specific vibrational modes, and reveal the distribution of molecular species in the single cell. These results were never imaged with the conventional IR microscope.