Open AccessMeasurement of the ratio of forward-propagating to back-propagating second harmonic signal using a single objective
Author(s) -
Xiaoxing Han,
Edward B. Brown
Publication year - 2010
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.010538
Subject(s) - optics , second harmonic generation , signal (programming language) , lens (geology) , confocal , materials science , harmonic , microscope , laser , physics , computer science , acoustics , programming language
In this paper, we present a method to determine, for the first time, the ratio of forward-propagating second harmonic (SHG) signal to back-propagating SHG signal (F/B) in vivo on the surface of intact tissue samples without any biopsy or tissue sectioning, using only epidetection (i.e., via a single objective lens). This method has the additional benefit of using the confocal detection apparatus already contained within common commercially available two-photon laser-scanning microscopes, and hence can allow the measurement of the SHG F/B ratio in vivo with minimal purchase of new equipment.