Measurement of the ratio of forward-propagating to back-propagating second harmonic signal using a single objective | Zendy

Xiaoxing Han | Zendy; Edward B. Brown | Zendy

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Measurement of the ratio of forward-propagating to back-propagating second harmonic signal using a single objective

Author(s) -

Xiaoxing Han,

Edward B. Brown

Publication year - 2010

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.18.010538

Subject(s) - optics , second harmonic generation , signal (programming language) , lens (geology) , confocal , materials science , harmonic , microscope , laser , physics , computer science , acoustics , programming language

In this paper, we present a method to determine, for the first time, the ratio of forward-propagating second harmonic (SHG) signal to back-propagating SHG signal (F/B) in vivo on the surface of intact tissue samples without any biopsy or tissue sectioning, using only epidetection (i.e., via a single objective lens). This method has the additional benefit of using the confocal detection apparatus already contained within common commercially available two-photon laser-scanning microscopes, and hence can allow the measurement of the SHG F/B ratio in vivo with minimal purchase of new equipment.

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