Open AccessA simple scanless two-photon fluorescence microscope using selective plane illumination
Author(s) -
Jonathan A. Palero,
Susana I. C. O. Santos,
David Artigas,
Pablo Loza-Álvarez
Publication year - 2010
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.008491
Subject(s) - optics , microscope , fluorescence microscope , two photon excitation microscopy , microscopy , fluorescence , materials science , multiphoton fluorescence microscope , optical microscope , optical sectioning , excited state , light sheet fluorescence microscopy , photon , physics , scanning electron microscope , atomic physics
We demonstrate a simple scanless two-photon (2p) excited fluorescence microscope based on selective plane illumination microscopy (SPIM). Optical sectioning capability is presented and depth-resolved imaging of cameleon protein in C. elegans pharyngeal muscle is implemented.