Open AccessGabor-based fusion technique for Optical Coherence Microscopy
Author(s) -
Jannick P. Rolland,
Panomsak Meemon,
Supraja Murali,
Kevin P. Thompson,
Kye-Sung Lee
Publication year - 2010
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.18.003632
Subject(s) - optics , optical coherence tomography , microscopy , lens (geology) , depth of field , image resolution , interferometry , resolution (logic) , image fusion , materials science , light sheet fluorescence microscopy , computer science , physics , artificial intelligence , scanning confocal electron microscopy , image (mathematics)
We recently reported on an Optical Coherence Microscopy technique, whose innovation intrinsically builds on a recently reported - 2 microm invariant lateral resolution by design throughout a 2 mm cubic full-field of view - liquid-lens-based dynamic focusing optical probe [Murali et al., Optics Letters 34, 145-147, 2009]. We shall report in this paper on the image acquisition enabled by this optical probe when combined with an automatic data fusion method developed and described here to produce an in-focus high resolution image throughout the imaging depth of the sample. An African frog tadpole (Xenopus laevis) was imaged with the novel probe and the Gabor-based fusion technique, demonstrating subcellular resolution in a 0.5 mm (lateral) x 0.5 mm (axial) without the need, for the first time, for x-y translation stages, depth scanning, high-cost adaptive optics, or manual intervention. In vivo images of human skin are also presented.