Open AccessIntensity sensitivity of gold nanostructures and its application for high-throughput biosensing
Author(s) -
Kuang-Li Lee,
Shu-Han Wu,
PeiKuen Wei
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.023104
Subject(s) - biosensor , surface plasmon , materials science , optics , light intensity , plasmon , intensity (physics) , sensitivity (control systems) , extraordinary optical transmission , nanostructure , surface plasmon resonance , optoelectronics , surface plasmon polariton , nanotechnology , physics , nanoparticle , engineering , electronic engineering
A new microarray for dynamical studies of surface biomolecular interactions without fluorescent labeling is proposed. We employed gold nanostructures to excite surface plasmons on the microarray surface and detected the intensity changes in the extraordinary transmission. The calculation and measurement results indicate that the nanoslit array has an intensity sensitivity much higher than the nanohole array due to its narrower resonant bandwidth. In addition, the sensitivity is increased as the slit width decreases. For 35 nm slit width, the intensity sensitivity reaches to approximately 4000%/RIU, two times larger than the slit width larger than 150 nm. Using the intensity changes, we demonstrate a 10 x 10 microarray for real-time measurements of antigen-antibody and DNA-DNA interactions.