Open AccessThree-color multiplex CARS for fast imaging and microspectroscopy in the entire CHn stretching vibrational region
Author(s) -
Jae Yong Lee,
Se-Hwa Kim,
Dae Won Moon,
Eun Seong Lee
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.022281
Subject(s) - optics , raman spectroscopy , femtosecond , materials science , raman scattering , sapphire , coherent anti stokes raman spectroscopy , multiplex , narrowband , laser , physics , bioinformatics , biology
We present a three-color multiplex coherent anti-Stokes Raman scattering (CARS) setup that facilitates a prompt recording of broadband CARS spectra along with a fast CARS imaging. With separate narrowband Stokes and probe beams being introduced in the near IR, we are able to incorporate a stable, wideband Ti:sapphire femtosecond laser as a pump beam that covers the full range of Raman shift for CHn stretching vibrational modes. Experimentally, high-resolution multiplex CARS signals are allowed to investigate molecular vibrations over the range of 2650 cm-1 - 3050 cm-1, which are spectrally integrated to construct lipid-sensitive images. It is demonstrated that the proposed implementation promises a particular benefit on CARS imaging of lipid-rich tissue structures by providing detailed information on CHn Raman-active vibrations at points of interest on the CARS images that can be obtained at high frame rates.