Open AccessHybrid shear force feedback/scanning quantitative phase microscopy applied to subsurface imaging
Author(s) -
Kert Edward,
Faramarz Farahi,
Robert J. Hocken
Publication year - 2009
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.018408
Subject(s) - phase imaging , optics , microscopy , materials science , decoupling (probability) , phase (matter) , shear (geology) , composite material , chemistry , physics , organic chemistry , control engineering , engineering
Quantitative phase microscopy allows for the study of the surface morphology and dynamics of transparent biological specimens. Although phase data often contains coupled subsurface information, decoupling the surface and subsurface components is often very difficult or impossible. We hereby present a simple procedure which exploits simultaneous obtained quantitative phase and shear-force feedback topography data to extract subsurface sample information. Our results reveal subsurface features in fabricated samples and fish erythrocytes.