Open AccessHigh-Q polymer-coated microspheres for immunosensing applications
Author(s) -
Silvia Soria,
Francesco Baldini,
Simone Berneschi,
Franco Cosi,
Ambra Giannetti,
G. Nunzi Conti,
S. Pelli,
Giancarlo C. Righini,
Bruno Tiribilli
Publication year - 2009
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.014694
Subject(s) - surface modification , materials science , microsphere , polymer , covalent bond , homogeneous , chemical engineering , resonator , nanotechnology , chemistry , organic chemistry , composite material , optoelectronics , physics , engineering , thermodynamics
Homogeneous polymeric thin layers have been used as functionalizing agents on silica microspherical resonators in view of the implementation of an immunosensor. We have characterized the microspheres functionalized with poly-L-lactic acid and Eudragit L100, as an alternative to the commonly used 3-Aminopropyltrimethoxysilane. It is shown that polymeric functionalization does not affect the high quality factor (Q greater than 10(7)) of the silica microspheres, and that the Q factor is about 3 x 10(5) after chemical activation and covalent binding of immunogammaglobulin (IgG). This functionalizing process of the microresonator constitutes a promising step towards the achievement of an ultra sensitive immunosensor.