Open AccessFourier transform-second-harmonic generation imaging of biological tissues
Author(s) -
Raghu Ambekar Ramachandra Rao,
Monal R. Mehta,
Kimani C. Toussaint
Publication year - 2009
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.014534
Subject(s) - optics , fourier transform , materials science , second harmonic generation , cornea , orientation (vector space) , spatial frequency , biomedical engineering , stack (abstract data type) , fourier analysis , harmonic , computer science , acoustics , physics , mathematics , medicine , laser , geometry , quantum mechanics , programming language
Fourier transform-second-harmonic generation imaging is employed to obtain quantitative metrics of collagen fibers in biological tissues. In particular, the preferred orientation and maximum spatial frequency of collagen fibers for selected regions of interest in porcine trachea, ear, and cornea are determined. These metrics remain consistent when applied to collagen fibers in the ear, which can be expected from observation. Collagen fibers in the trachea are more random with large standard deviations in orientation, and large variations in maximum spatial frequency. In addition, these metrics are used to investigate structural changes through a 3D stack of the cornea. This technique can be used as a quantitative marker to assess the structure of collagen fibers that may change due to damage from disease or physical injury.