Open AccessLight scattering characterization of mitochondrial aggregation in single cells
Author(s) -
Xuantao Su,
Kamal Deep Singh,
W. Rozmus,
C. Backhouse,
C. E. Capjack
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.013381
Subject(s) - scattering , optics , light scattering , finite difference time domain method , physics , fourier transform , forward scatter , characterization (materials science) , mitochondrion , computational physics , chemistry , quantum mechanics , biochemistry
Three dimensional finite-difference time-domain (FDTD) simulations are employed to show that light scattering techniques may be used to infer the mitochondrial distributions that exist within single biological cells. Two-parameter light scattering plots of the FDTD light scattering spectra show that the small angle forward scatter can be used to differentiate the case of a random distribution of mitochondria within a cell model from that in which the mitochondria are aggregated to the nuclear periphery. Fourier transforms of the wide angle side scatter spectra show a consistent highest dominant frequency, which may be used for size differentiation of biological cells with distributed mitochondria.