Open AccessSimplified approach to diffraction tomography in optical microscopy
Author(s) -
Reto Fiolka,
Kai Wicker,
Rainer Heintzmann,
Andreas Stemmer
Publication year - 2009
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.012407
Subject(s) - optics , wavefront , diffraction tomography , diffraction , interferometry , tomography , scattering , physics , tomographic reconstruction , iterative reconstruction , optical tomography , microscopy , computer science , computer vision
We present a novel microscopy technique to measure the scattered wavefront emitted from an optically transparent microscopic object. The complex amplitude is decoded via phase stepping in a common-path interferometer, enabling high mechanical stability. We demonstrate theoretically and practically that the incoherent summation of multiple illumination directions into a single image increases the resolving power and facilitates image reconstruction in diffraction tomography. We propose a slice-by-slice object-scatter extraction algorithm entirely based in real space in combination with ordinary z-stepping. Thereby the computational complexity affiliated with tomographic methods is significantly reduced. Using the first order Born approximation for weakly scattering objects it is possible to obtain estimates of the scattering density from the exitwaves.