Open AccessThree-dimensional holographic imaging of living tissue using a highly sensitive photorefractive polymer device
Author(s) -
Michaël Salvador,
J. Prauzner,
Sebastian Köber,
Klaus Meerholz,
John Turek,
Kwan Jeong,
David D. Nolte
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.011834
Subject(s) - holography , optics , photorefractive effect , optical coherence tomography , coherence (philosophical gambling strategy) , light field , holographic interferometry , digital holography , materials science , holographic data storage , holographic display , physics , quantum mechanics
Photorefractive materials are dynamic holographic storage media that are highly sensitive to coherent light fields and relatively insensitive to a uniform light background. This can be exploited to effectively separate ballistic light from multiply-scattered light when imaging through turbid media. We developed a highly sensitive photorefractive polymer composite and incorporated it into a holographic optical coherence imaging system. This approach combines the advantages of coherence-domain imaging with the benefits of holography to form a high-speed wide-field imaging technique. By using coherence-gated holography, image-bearing ballistic light can be captured in real-time without computed tomography. We analyzed the implications of Fourier-domain and image-domain holography on the field of view and image resolution for a transmission recording geometry, and demonstrate holographic depth-resolved imaging of tumor spheroids with 12 microm axial and 10 microm lateral resolution, achieving a data acquisition speed of 8 x 10(5) voxels/s.