Open AccessSecond harmonic microscopy of axonemes
Author(s) -
Christophe Odin,
Claire Heichette,
Denis Chrétien,
Yann Le Grand
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.009235
Subject(s) - optics , microscopy , polarization (electrochemistry) , second harmonic generation , physics , laser , chemistry
We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility chizxx/chixzx = 1.1+/-0.2 and chizzz/chixzx = 4+/-0.5 of axonemes.