Open AccessDigital In-line Holography with femtosecond VUV radiation provided by the free-electron laser FLASH
Author(s) -
Axel Rosenhahn,
Florian Staier,
Thomas Nisius,
David E. Schafer,
Ruth Barth,
Christof Christophis,
Lorenz-M. Stadler,
S. Streit-Nierobisch,
Christian Gutt,
Adrian P. Mancuso,
Andreas Schropp,
J. Gulden,
B. Reime,
J. Feldhaus,
E. Weckert,
Bastian Pfau,
C. Günther,
René Könnecke,
Stefan Eisebitt,
M. Martins,
B. Faatz,
N. Guerassimova,
K. Honkavaara,
R. Treusch,
E.L. Saldin,
S. Schreiber,
E.A. Schneidmiller,
M.V. Yurkov,
Ivan A. Vartanyants,
G. Grübel,
M. Grunze,
Thomas Wilhein
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.008220
Subject(s) - optics , femtosecond , laser , pinhole (optics) , holography , radiation , materials science , physics , optoelectronics
Femtosecond vacuum ultraviolet (VUV) radiation provided by the free-electron laser FLASH was used for digital in-line holographic microscopy and applied to image particles, diatoms and critical point dried fibroblast cells. To realize the classical in-line Gabor geometry, a 1 microm pinhole was used as spatial filter to generate a divergent light cone with excellent pointing stability. At a fundamental wavelength of 8 nm test objects such as particles and diatoms were imaged at a spatial resolution of 620 nm. In order to demonstrate the applicability to biologically relevant systems, critical point dried rat embryonic fibroblast cells were for the first time imaged with free-electron laser radiation.