Open AccessAutofocusing and edge detection schemes in cell volume measurements with quantitative phase microscopy
Author(s) -
Seungrag Lee,
Ji Yong Lee,
Wenzhong Yang,
Dug Young Kim
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.006476
Subject(s) - optics , focus (optics) , microscopy , volume (thermodynamics) , materials science , enhanced data rates for gsm evolution , edge detection , digital holographic microscopy , displacement (psychology) , phase (matter) , position (finance) , computer science , image processing , physics , computer vision , psychology , finance , quantum mechanics , economics , image (mathematics) , psychotherapist
We have proposed and demonstrated a very sensitive volume measurement scheme for a live cell with a quantitative phase microscopy (QPM) utilizing auto-focusing and numerical edge detection schemes. An auto-focusing technique with two different focus measures is applied to find the focus dependent errors in our live cell volume measurement system. The volume of a polystyrene bead sample with 3 mum diameter has been measured for the validity test of our proposed method. We have shown that a small displacement of an object from its focusing position can cause a large volume error. A numerical edge detection technique is also used to accurately resolve the boundary between a cell and its suspension medium. We have applied this method to effectively suppress errors by the surrounding medium of a single red blood cell (RBC).