Open AccessMultiplexed force measurements on live cells with holographic optical tweezers
Author(s) -
Cecile O. Mejean,
A. Schaefer,
Eleanor A. Millman,
Paul Forscher,
Eric R. Dufresne
Publication year - 2009
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.17.006209
Subject(s) - optical tweezers , holography , multiplexing , optics , bead , suspension (topology) , optical force , materials science , adhesion , coupling (piping) , tweezers , nanotechnology , biophysics , physics , biology , computer science , telecommunications , mathematics , homotopy , pure mathematics , metallurgy , composite material
We describe open-loop and closed-loop multiplexed force measurements using holographic optical tweezers. We quantify the performance of our novel video-based control system in a driven suspension of colloidal particles. We demonstrate our system's abilities with the measurement of the mechanical coupling between Aplysia bag cell growth cones and beads functionalized with the neuronal cell adhesion molecule, apCAM. We show that cells form linkages which couple beads to the underlying cytoskeleton. These linkages are intermittent, stochastic and heterogeneous across beads distributed near the leading edge of a single growth cone.