Open AccessFocal modulation microscopy
Author(s) -
Nanguang Chen,
Chee-Howe Wong,
Colin J. R. Sheppard
Publication year - 2008
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.018764
Subject(s) - optics , microscopy , light sheet fluorescence microscopy , materials science , optical sectioning , resolution (logic) , diffraction , image resolution , fluorescence microscope , fluorescence , fluorescence lifetime imaging microscopy , biological imaging , biological specimen , photoactivated localization microscopy , two photon excitation microscopy , modulation (music) , light scattering , scattering , microscope , excited state , scanning confocal electron microscopy , super resolution microscopy , physics , artificial intelligence , computer science , acoustics , nuclear physics
We report a novel light microscopy method for high resolution molecular imaging of thick biological tissues with one photon excited fluorescence. Effective optical sectioning and diffraction limited spatial resolution are achieved when imaging deep inside a multiple-scattering medium by the use of focal modulation, a technique for suppressing the background fluorescence signal excited by scattered light. Our method has been validated with animal tissue and an imaging depth around 600 microns has been demonstrated.