Open AccessQuantitative interpretations of Visible-NIR reflectance spectra of blood
Author(s) -
Yulia M. Serebrennikova,
Jennifer Smith,
Debra E. Huffman,
Germán F. Leparc,
Luis H. GarcíaRubio
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.018215
Subject(s) - optics , mie scattering , scattering , wavelength , absorption (acoustics) , diffuse reflection , photon diffusion , spectral line , calibration , light scattering , materials science , diffusion , refractive index , physics , light source , quantum mechanics , astronomy , thermodynamics
This paper illustrates the implementation of a new theoretical model for rapid quantitative analysis of the Vis-NIR diffuse reflectance spectra of blood cultures. This new model is based on the photon diffusion theory and Mie scattering theory that have been formulated to account for multiple scattering populations and absorptive components. This study stresses the significance of the thorough solution of the scattering and absorption problem in order to accurately resolve for optically relevant parameters of blood culture components. With advantages of being calibration-free and computationally fast, the new model has two basic requirements. First, wavelength-dependent refractive indices of the basic chemical constituents of blood culture components are needed. Second, multi-wavelength measurements or at least the measurements of characteristic wavelengths equal to the degrees of freedom, i.e. number of optically relevant parameters, of blood culture system are required. The blood culture analysis model was tested with a large number of diffuse reflectance spectra of blood culture samples characterized by an extensive range of the relevant parameters.