Open AccessThe divided aperture technique for microscopy through scattering media
Author(s) -
Colin J. R. Sheppard,
Wei Gong,
Ke Si
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.017031
Subject(s) - optics , microscopy , materials science , diffraction , resolution (logic) , confocal microscopy , aperture (computer memory) , optical microscope , specular reflection , scattering , light sheet fluorescence microscopy , numerical aperture , light scattering , interference microscopy , microscope , scanning confocal electron microscopy , physics , scanning electron microscope , wavelength , computer science , artificial intelligence , acoustics
A diffraction analysis is presented for image formation in confocal microscopy using the divided aperture technique, which uses two D-shaped apertures (also called specular microscopy). The effects of increasing the width of a divider, that separates the two D shapes, are investigated. As the width is increased, the resolution degrades. The efficiency of singly-scattered light rejection is not improved with increased width.