Open AccessDelivery of sub-10-fs pulses for nonlinear optical microscopy by polarization-maintaining single mode optical fiber
Author(s) -
Adam M. Larson,
Alvin T. Yeh
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.014723
Subject(s) - optics , materials science , polarization mode dispersion , multi mode optical fiber , polarization maintaining optical fiber , dispersion shifted fiber , optical fiber , second harmonic generation , polarization (electrochemistry) , single mode optical fiber , fiber laser , nonlinear optics , microscopy , laser , optoelectronics , physics , fiber optic sensor , chemistry
Broadband, sub-10-fs pulses, can be propagated through polarization-maintaining single mode fiber (PMF) for use in nonlinear optical microscopy (NLOM). We demonstrate delivery of near transform-limited, 1 nJ pulses from a Ti:Al(2)O(3) (75 MHz repetition rate) oscillator via PMF to the NLOM focal plane while maintaining 120 nm of bandwidth. Negative group delay dispersion (GDD) introduced to pre-compensate normal dispersion of the optical fiber and microscope optics ensured linear pulse propagation through the PMF. The minimized time-bandwidth product of the laser pulses at the NLOM focus allowed the nonlinear excitation of multiple fluorophores simultaneously without central wavelength tuning. Polarization sensitive NLOM imaging using second harmonic generation in collagen was demonstrated using PMF delivered pulses. Two-photon excited fluorescence spectra and second harmonic images taken with and without the fiber indicates that the fiber based system is capable of generating optical signals that are within a factor of two to three of our traditional NLOM.