Open AccessLow-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology
Author(s) -
Toyohiko Yamauchi,
Hidenao Iwai,
Makoto Miwa,
Yutaka Yamashita
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.012227
Subject(s) - interference microscopy , nanometre , optics , microscope , phase imaging , materials science , interference (communication) , microscopy , optical microscope , phase (matter) , physics , scanning electron microscope , channel (broadcasting) , electrical engineering , quantum mechanics , engineering
We have developed a Linnik-type interference microscope provided with a low-coherent light source to obtain topographic images of an intact cellular membrane on a nanometer scale. Our technique is based on measurement of the interference between light reflected from the cell surface and a reference beam. The results show full field surface topography of cultured cells and reveal an intrinsic membrane motion of tens of nanometers.