Open AccessAlgorithm and simulation for analysis of bio-images obtained by aperture diffraction based optical MEMS
Author(s) -
Xiaodong Zhou,
Daniel Puiu Poenar,
Kai Yu Liu,
Man Siu Tse,
Chew-Kiat Heng,
Swee Ngin Tan,
Nan Zhang
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.011937
Subject(s) - optics , diffraction , microelectromechanical systems , aperture (computer memory) , refractive index , materials science , ptychography , position (finance) , optoelectronics , physics , acoustics , finance , economics
This paper proposes a novel method to detect transparent living cells in a transparent microfluidic chamber by optical diffraction of an aperture or an aperture array. Through the analysis of the far-field diffraction pattern, one of the parameters of the cells, including the size, refractive index, or position, can be extracted by the analysis software developed in this paper. Calculations are carried out to discuss the key issues of this MEMS device, and our simulation is verified by diffraction patterns of transparent microparticles on fabricated apertures, recorded via a digital camera.