Open AccessSimple and robust image-based autofocusing for digital microscopy
Author(s) -
Siavash Yazdanfar,
Kevin B. Kenny,
Krenar Tasimi,
Alex D. Corwin,
Elizabeth L. Dixon,
Robert J. Filkins
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.008670
Subject(s) - optics , microscopy , focus (optics) , digital imaging , image processing , digital image processing , digital image , fluorescence microscope , computer science , computer vision , microscope , artificial intelligence , sample (material) , materials science , point spread function , digital holographic microscopy , image (mathematics) , fluorescence , physics , thermodynamics
A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few as two intermediate images to bring the sample into focus. The algorithm is adapted to a commercial inverted microscope and used to automate brightfield and fluorescence imaging of histopathology tissue sections.