Open AccessDynamics of femtosecond laser photo-modification of collagen fibers
Author(s) -
Vladimir A. Hovhannisyan,
Wen Lo,
Chieh Hu,
Shean-Jen Chen,
Dong Chen
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.007958
Subject(s) - femtosecond , materials science , laser , autofluorescence , optics , second harmonic generation , confocal , surface modification , cornea , biophysics , fluorescence , chemistry , physics , biology
In this work, we investigate the non-ablative, non-thermal photo-modification of collagen fibers by femtosecond Ti:Sa laser. The effect was induced and simultaneously registered during the repetitive laser scanning of type I collagen (rat tail and bovine Achilles' tendon), and bovine cornea. An irreversible increase in two-photon autofluorescence and a decrease in second harmonic generation intensities were associated with the collagen femtosecond laser photo-modification. Confocal spectral imaging revealed the formation of new fluorescent species. Controllable nonlinear photo-modification of collagen fibers and bovine cornea with approximately 2 microm spatial resolution was demonstrated.