Open AccessMicrofluidic CARS cytometry
Author(s) -
Hanwei Wang,
Ning Bao,
Thuc T. Le,
Chang Lu,
JiXin Cheng
Publication year - 2008
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.005782
Subject(s) - cytometry , flow cytometry , materials science , microfluidics , polydimethylsiloxane , raman scattering , population , microscopy , microscope , optics , polystyrene , raman spectroscopy , nanotechnology , biology , physics , microbiology and biotechnology , demography , sociology , composite material , polymer
Coherent anti-stokes Raman scattering (CARS) flow cytometry was demonstrated by combining a laser-scanning CARS microscope with a polydimethylsiloxane (PDMS) based microfluidic device. Line-scanning across the hydrodynamically focused core stream was performed for detection of flowing objects. Parameters were optimized by utilizing polystyrene beads as flowing particles. Population measurements of adipocytes isolated from mouse fat tissues demonstrated the viability of microfluidic CARS cytometry for quantitation of adipocyte size distribution. CARS cytometry could be a new modality for quantitative analysis with vibrational selectivity.