Open AccessQuantification of in vivo autofluorescence dynamics during renal ischemia and reperfusion under 355 nm excitation
Author(s) -
Rajesh N. Raman,
Christopher D. Pivetti,
Dennis L Matthews,
Christoph Troppmann,
Stavros G. Demos
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.004930
Subject(s) - autofluorescence , in vivo , ischemia , renal ischemia , kidney , ex vivo , pathology , materials science , biomedical engineering , optics , medicine , reperfusion injury , cardiology , physics , fluorescence , biology , microbiology and biotechnology
We explore a method to quantitatively assess the ability of in vivo autofluorescence as a means to quantify the progression of longer periods of renal warm ischemia and reperfusion in a rat model. The method employs in vivo monitoring of tissue autofluorescence arising mainly from NADH as a means to probe the organ's function and response to reperfusion. Clinically relevant conditions are employed that include exposure of the kidney to ischemia on the order of tens of minutes to hours. The temporal profile during the reperfusion phase of the autofluorescence intensity averaged over an area as large as possible was modeled as the product of two independent exponential functions. Time constants were extracted from fits to the experimental data and their average values were found to increase with injury time.