Open AccessQuantitative bioluminescence tomography guided by diffuse optical tomography
Author(s) -
Qizhi Zhang,
Lu Yin,
Yiyong Tan,
Zhen Yuan,
Huabei Jiang
Publication year - 2008
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.001481
Subject(s) - diffuse optical imaging , bioluminescence , optics , tomography , optical tomography , materials science , physics , chemistry , biochemistry
We present for the first time experimental evidence that the quantitative accuracy of bioluminescence tomography (BLT) can be significantly improved by incorporating prior spatial distribution of optical properties of heterogeneous media obtained from diffuse optical tomography (DOT). A series of experiments were conducted using a CCD-based scanning system where millimeter-size bioluminescent targets were embedded in a 3 x 3 x 5 cm optically heterogeneous scattering medium. The results show that the BLT images with the recovered optical property distribution in place are considerably better reconstructed compared to that without such prior information, in terms of the location, size and source strength of the targets.