Open AccessDepth-resolved optical imaging of transmural electrical propagation in perfused heart
Author(s) -
Elizabeth M. C. Hillman,
Olivier Bernus,
Emily C. Pease,
Michael J. Bouchard,
Arkady M. Pertsov
Publication year - 2007
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.017827
Subject(s) - optical mapping , ventricle , optics , laminar flow , wave propagation , optical recording , physics , materials science , biomedical engineering , medicine , cardiology , mechanics
We present a study of the 3-dimensional (3D) propagation of electrical waves in the heart wall using Laminar Optical Tomography (LOT). Optical imaging contrast is provided by a voltage sensitive dye whose fluorescence reports changes in membrane potential. We examined the transmural propagation dynamics of electrical waves in the right ventricle of Langendorf perfused rat hearts, initiated either by endo-cardial or epi-cardial pacing. 3D images were acquired at an effective frame rate of 667Hz. We compare our experimental results to a mathematical model of electrical transmural propagation. We demonstrate that LOT can clearly resolve the direction of propagation of electrical waves within the cardiac wall, and that the dynamics observed agree well with the model of electrical propagation in rat ventricular tissue.