Quantitative polarized phase microscopy for birefringence imaging | Zendy

Nicoleta Dragomir | Zendy; Xiao Ming Goh | Zendy; Claire L. Curl | Zendy; Lea M.D. Delbridge | Zendy; Ann Roberts | Zendy

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Quantitative polarized phase microscopy for birefringence imaging

Author(s) -

Nicoleta Dragomir,

Xiao Ming Goh,

Claire L. Curl,

Lea M.D. Delbridge,

Ann Roberts

Publication year - 2007

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.15.017690

Subject(s) - birefringence , optics , phase imaging , materials science , microscopy , phase (matter) , characterization (materials science) , anisotropy , polarized light microscopy , optical microscope , physics , scanning electron microscope , quantum mechanics

A novel application of quantitative phase imaging under linearly polarized light is introduced for studying unstained anisotropic live cells. The method is first validated as a technique for mapping the two-dimensional retardation distribution of a well-characterized optical fiber and is then applied to the characterization of unstained isolated cardiac cells. The experimental retardation measurements are in very good agreement with the established Brace-Köhler method, and additionally provide spatially resolved cell birefringence and phase data.

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