Open AccessFluorescence ratio thermometry in a microfluidic dual-beam laser trap
Author(s) -
Susanne Ebert,
Kort Travis,
Bryan Lincoln,
Jochen Guck
Publication year - 2007
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.015493
Subject(s) - materials science , optics , trap (plumbing) , laser induced fluorescence , microscope , laser , rhodamine , laser power scaling , confocal , fluorescence , wavelength , beam (structure) , microfluidics , rhodamine b , optoelectronics , physics , nanotechnology , chemistry , biochemistry , photocatalysis , meteorology , catalysis
The dual-beam laser trap is a versatile tool with many possible applications. In order to characterize its thermal properties in a microfluidic trap geometry we have developed a non-intrusive fluorescence ratio technique using the temperature sensitive dye Rhodamine B and the temperature independent reference dye Rhodamine 110. We measured temperature distribution profiles in the trap with submicron spatial resolution on a confocal laser-scanning microscope. The maximum heating in the center of the trap amounts to (13 +/- 2) degrees C/W for a wavelength of lambda = 1064 nm and scales linearly with the applied power. The measurements correspond well with simulated temperature distributions.